Alkaline Phosphatase Kit

Alkaline Phosphatase Kit

Introduction:

• AutoZyme Alkaline Phosphatase is a reagent set for determination of Alkaline Phosphatase activity based on kinetic method using p-nitrophenyl phosphate (pNPP).

• AutoZyme Alkaline Phosphatase is a single reagent system, using one step procedure.

• AutoZyme Alkaline Phosphatase has one step reconstitution. It involves dissolving Substrate tablet in Diluent

• AutoZyme Alkaline Phosphatase is a High Stability Reagent.

• AutoZyme Alkaline Phosphatase is linear upto 700 lull.

• Alkaline Phosphatase activity can be determined in just 215 minutes.

• AutoZyme Alkaline Phosphatase can be used on any Spectrophotometer, Discrete semlautomated and Automated analyzer. Programme can be designed for any specific analyzer upon request.
  
  Principle:

  Alkaline Phosphatase cleaves p-nitrophenyl phosphate (pNPP) into p-nitrophenol and phosphatase. p-nitrophenol is a yellow color compound in alkaline medium and absorbs light at 405 nm. The rate of increase in absorbance at 405 nm. is proportional to Alkaline phosphatase activity in specimen.

Specimen Collection & Preservation:

  Blood should be collected In a clean dry container. Haemolyzed specimen should be avoided as it may falsely elevate results. EDTA, Citrate and Oxalate inhibit Alkaline Phosphatase activity and should not be used as anticoagulant.

For plasma separation any of the following two anticoagulants may be used

• HEPARIN 200 ml blood

• SODIUM FLUORIDE 10 mg/ml blood
  
  Serum /plasma should be separated immediately from cells. Alkaline Phosphatase is stable for 4 days at 2 - 8°C and several months when stored at -10°C.

• Available Products:

MOQ: 50 to 100 kits.